引用本文: 韩尚明，李文豪，李惠霞，张译文，倪春辉. 甘肃省孢囊线虫新记录种: 艾球孢囊线虫. 欢乐牛牛, 2020, 37(12): 1-7 doi: shu

Citation: HAN S M, LI W H, LI H X, ZHANG Y W, NI C H. , a new cyst nematode record in Gansu, China. Pratacultural Science, 2020, 37(12): 1-7 doi: shu

甘肃省孢囊线虫新记录种: 艾球孢囊线虫

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gansunongyedaxuezhiwubaohuxueyuan/gansushengnongzuowubingchonghaishengwufangzhigongchengshiyanshi，gansu lanzhou 730070

作者简介: 韩尚明(1995-)，男，甘肃环县人，在读硕士生，研究方向为植物病原线虫学。E-mail: ;

通讯作者: 李惠霞, .cn

基金项目:亚博滚球国家公益性行业(农业)科研专项(201503114)；甘肃省科技支撑计划(1604NKCA053-2)

摘要: 在2018 − 2019年，在甘肃省天祝县高寒草地艾蒿(Artemisia argyi)根际分离得到1个孢囊线虫群体，采用形态学结合分子生物学技术进行了种类鉴定。该孢囊线虫群体形态特征: 孢囊呈褐色，球形，颈部较为突出，无阴门锥；阴门膜孔为环膜孔型，无下桥和泡状突。二龄幼虫线形，头部与体驱间缢缩不明显，口针强壮。尾部圆锥形，末端较尖锐，经形态学初步鉴定为艾球孢囊线虫(Globodera artemisiae)。对该群体ITS-rDNA和28S-rRNA D2-D3区进行扩增，分别得到948 bp和1 015 bp两个片段。测序后进行聚类分析，发现该群体与艾球孢囊线虫聚为一支，置信度为97%以上。结合形态学和分子生物学特征，将该群体鉴定为艾球孢囊线虫，这也是甘肃首次报道球孢囊线虫属线虫，系甘肃省新记录种。

关键词:

English

Globodera artemisiae, a new cyst nematode record in Gansu, China

college of plant protection, gansu agricultural university/biocontrol engineering laboratory of crop diseases and pests of gansu province, lanzhou 730070, gansu, china

Corresponding author: Huixia LI, .cn

Received Date: 2019-03-30
Available Online: 2019-12-15

Abstract: In 2018 − 2019, a cyst nematode population was isolated from the rhizosphere of Artemisia argyi on an alpine grassland in Tianzhu County, Gansu Province, China. The morphological characteristics of this nematode population were: brown spherical cysts, a prominent neck, generally circumfenestrate, with no vulval cone, underbridge, or bullae. Second-stage juveniles were vermiform, with inconspicuous head constriction, and a strong stylet. The tail was conical and sharply pointed. The ITS-rDNA and 28S-rRNA D2-D3 regions of the population were amplified, from which 948 and 1 015 bp fragments were obtained, respectively. After sequencing, cluster analysis showed that the population was clustered as Globodera artemisiae, with a confidence of over 97%. This population was identified as G. artemisiae based on its morphological and molecular characteristics. This is the first report of the genus Globodera and a new species record in Gansu Province.

Key words:

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图 1 甘肃艾蒿球孢囊线虫显微照片

Figure 1. Light micrographs of Globodera artemisiae from Gansu

下载: 全尺寸图片幻灯片

图 2 亚博滚球基于最大似然法构建的艾蒿群体ITS-rDNA部分序列系统进化树

Figure 2. 亚博滚球 Phylogenetic tree of partial ITS-rDNA sequences of a mugwort population based on the maximum likelihood method

下载: 全尺寸图片幻灯片

图 3 亚博滚球基于最大似然法构建的艾蒿群体28S-rRNA D2-D3区序列系统进化树

Figure 3. Phylogenetic tree of partial 28S-rRNA D2-D3 sequences of a mugwort population based on the maximum likelihood method

下载: 全尺寸图片幻灯片

表 1 艾蒿根际孢囊线虫的形态测量值

Table 1. Morphometrics of cyst nematodes from the rhizosphere of Artemisia argyi

群体 Species population	甘肃艾蒿群 Population of Artemisia argyi	陈品三等^[19]报道 Reported by Chen et al.^[19]	Dobosz等^[20]报道 Reported by Dobosz et al.^[20]	Ogawa等^[21]报道 Reported by Ogawa et al.^[21]
孢囊长 Cyst length	526.4 (471.2～632.8)	500	524 (384～640)	−
孢囊宽 Cyst width	468.7 (371.6～524.4)	377.7	425 (316～696)	448.1 (320～620)
阴门膜孔长 Fenestral length	26.4 (24.2～27.8)	27.2	−	20.9 (16～27)
阴门膜孔宽 Fenestral width	24.5 (19.1～28.7)	25	−	18.7 (15～25)
二龄幼虫长 Juvenile length	466.4 (425.5～496.7)	476.5	461 (425～490)	460.6 (410～510)
二龄幼虫宽 Juvenile width	20.7 (17.4～22.0)	20.1	18 (20～24)
口针长 Stylet length	22.8 (20.4～24.5)	23.9	−	24.6 (24～25)
尾长 Tail length	47.6 (32.4～59.1)	47.2	55 (46～66)	47.2
透明尾长 Hyaline region	24.9 (20.8～27.6)	25.4	−	23.6

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甘肃省孢囊线虫新记录种: 艾球孢囊线虫

作者简介: 韩尚明(1995-)，男，甘肃环县人，在读硕士生，研究方向为植物病原线虫学。E-mail: ;

通讯作者: 李惠霞, .cn

English

Globodera artemisiae, a new cyst nematode record in Gansu, China

Corresponding author: Huixia LI, .cn

亚博滚球

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