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马利娟 范精琦 郗娜仁高娃 祁智 张林刚

引用本文: 马利娟,范精琦,郗娜仁高娃,祁智,张林刚. 内蒙古两种生态型羊草叶绿体色素含量以及形态特征的对比. 欢乐牛牛, 2020, 37(0): 1-8 doi: 10.11829/j.issn.1001-0629.2020-0029 shu
Citation:  MA L J, FAN J Q, XI Narengaowa, Qi Z, ZHANG L G. Comparative study on chloroplast pigment concentration and morphology between two ecotypes of in Inner Mongolia. Pratacultural Science, 2020, 37(0): 1-8 doi: 10.11829/j.issn.1001-0629.2020-0029 shu

内蒙古两种生态型羊草叶绿体色素含量以及形态特征的对比

    作者简介: 马利娟(1994—),女,山西大同人,在读硕士生,研究方向为叶绿体发育。E-mail: ;
    通讯作者: 张林刚, lg-
  • 基金项目: 国家自然基金项目“GPA1和THF1协同调控植物气孔保卫细胞大小的机理研究”(31770261);国家自然基金地区项目“VIPP1蛋白运动的调控及其修复叶绿体被膜的分子机理研究”(31660062)

摘要: 羊草(Leymus chinensis)是在内蒙古草原上广泛分布的一种优良牧草,根据叶片颜色可以分为灰绿羊草和黄绿羊草。在逆境胁迫下,灰绿羊草较黄绿羊草具有更高的光合能力和更强的环境适应性,但是其机理还不清楚。本研究选取内蒙古呼和塔拉草原的灰绿羊草和黄绿羊草,测定其叶片叶绿素和类胡萝卜素的含量,分析两种生态型羊草叶片叶绿体数目、大小和形态结构特征,以及叶绿体光合蛋白复合体的差异。结果表明,灰绿羊草叶片叶绿素和类胡萝卜素含量均显著高于黄绿羊草(P < 0.01);单个细胞中叶绿体数目黄绿羊草显著高于(P < 0.01)灰绿羊草,但是叶绿体体积以及单个叶绿体中基粒类囊体的数目灰绿羊草显著大于黄绿羊草(P < 0.01);此外,黄绿羊草叶片不同细胞中含有两种形态结构不同的叶绿体;BN-PAGE(蓝绿温和胶)的结果显示,灰绿羊草叶绿体光系统Ⅰ(PSⅠ)和光系统Ⅱ(PSⅡ)二聚体的含量高于黄绿羊草。所以,较高的叶绿素、类胡萝卜素以及PSⅠ和PSⅡ蛋白复合体含量是灰绿羊草高效光合作用和更强环境适应能力的重要基础。

English

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  • 欢乐牛牛

    图 1  黄绿羊草和灰绿羊草叶片叶绿体的形态结构特征

    Figure 1.  Morphology and structure of chloroplasts in the leaves of gray-green and yellow-green Leymus chinensis ecotypes

    图 2  灰绿羊草和黄绿羊草叶绿体光合蛋白复合体的BN-PAGE分析结果

    Figure 2.  Photosynthetic protein complexes of gray-green grass and yellow-green grass separated with blue native gel electrophoresis

    表 1  欢乐牛牛 两种生态型羊草叶绿素和类胡萝卜素含量

    Table 1.  Concentrations of chlorophyll and carotenoids in the leaves of two ecotypic Leymus chinensis

    羊草类型
    Ecotype
    叶绿素a
    Chl a /(mg·g–1)
    叶绿素b
    Chl b /(mg·g–1)
    叶绿素a/b
    Chl a/b
    叶绿素a + b
    Chl a + b/(mg·g–1)
    类胡萝卜素
    Carotenoids/(mg·g–1)
    黄绿羊草
    Yellow-green grass
    2.69 ± 0.200.79 ± 0.043.39 ± 0.153.48 ± 0.230.59 ± 0.06
    灰绿羊草
    Gray-green grass
    3.97 ± 0.36*1.18 ± 0.13**3.36 ± 0.235.16 ± 0.46**0.77 ± 0.08**
     *和**分别表示灰绿羊草和黄绿羊草间显著性差异(P < 0.05)和极显著性差异(P < 0.01)。
     Chl: chlorophyll. * and ** indicate significant differences between gray-green grass and yellow-green grass at 0.05 and 0.01 levels, respectively.
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        • 通讯作者:  张林刚, lg-
        • 收稿日期:  2019-01-20
        • 网络出版日期:  2019-08-05
        通讯作者: 陈斌,
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